Learning about protein solubility from bacterial inclusion bodies
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چکیده
منابع مشابه
Learning about protein solubility from bacterial inclusion bodies
The progressive solving of the conformation of aggregated proteins and the conceptual understanding of the biology of inclusion bodies in recombinant bacteria is providing exciting insights on protein folding and quality. Interestingly, newest data also show an unexpected functional and structural complexity of soluble recombinant protein species and picture the whole bacterial cell factory sce...
متن کاملPackaging protein drugs as bacterial inclusion bodies for therapeutic applications
A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tissue engineering. Being fully biocompatible and with tuneable bio-physical prope...
متن کاملProtein aggregation as bacterial inclusion bodies is reversible.
Inclusion bodies are refractile, intracellular protein aggregates usually observed in bacteria upon targeted gene overexpression. Since their occurrence has a major economical impact in protein production bio-processes, in vitro refolding strategies are under continuous exploration. In this work, we prove spontaneous in vivo release of both beta-galactosidase and P22 tailspike polypeptides from...
متن کاملIsolation of biologically active nanomaterial (inclusion bodies) from bacterial cells
BACKGROUND In recent years bacterial inclusion bodies (IBs) were recognised as highly pure deposits of active proteins inside bacterial cells. Such active nanoparticles are very interesting for further downstream protein isolation, as well as for many other applications in nanomedicine, cosmetic, chemical and pharmaceutical industry.To prepare large quantities of a high quality product, the who...
متن کاملBacterial Inclusion Bodies Contain Amyloid-Like Structure
Protein aggregation is a process in which identical proteins self-associate into imperfectly ordered macroscopic entities. Such aggregates are generally classified as amorphous, lacking any long-range order, or highly ordered fibrils. Protein fibrils can be composed of native globular molecules, such as the hemoglobin molecules in sickle-cell fibrils, or can be reorganized beta-sheet-rich aggre...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2009
ISSN: 1475-2859
DOI: 10.1186/1475-2859-8-4